1. Pharmacology – Mechanism of drug action, in-vitro assays using 2D/3D cell culture models, drug-enzyme/receptor interactions & drug metabolism
a. Toxicology – Cytochrome P450 iso-enzymes Assays
The drug development from new chemical entities to pharmaceuticals is gaining momentum due to increasing pace of technology development in toxicology and clinical safety evaluation, albeit slowly. In vitro approaches, are extensively used in the preclinical and early clinical phases of drug development.
Stem cells isolation, characterization and culturing
Stem cells are a class of undifferentiated cells that have to ability to differentiate into specialized cell types. Main sources of stem cells are, embryos formed during the blastocyst phase of embryological development (embryonic stem cells) and adult tissues (adult stem cells).
b. Pharmaceutics and Analysis
Concentration of pharmaceutical entity and its metabolites, structure identification, validation and formulation
It is a process or processes to identify or quantify a substance or drug, the ingradeints of pharmaceutical preparation or mixture or the elucidation of structure of chemical constituents used in the formulation or product.
2. Technologies used:
PCR, qPCR, ELISA, Western Blot, Northern and Southern Blots, Cloning, Spectrophotometry, Cell culture (2D/3D models), Electrophoresis, Cryopreservation, & Exome sequencing, Chromatography(HPLC, GC, IR, Atomic Absorption Spectrometry), Flow Cytometry (through partners)